Construct: shRNA TRCN0000256749

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_058182.4-686s21c1
DNA Barcode:
CGTCTGTAATCCCAGCTACTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SMIM11A (54065)
Gene Description:
small integral membrane protein 11A
Transcript:
RefSeq NM_058182.4 (CURRENT)
Match location:
Position 686 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000256749)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTCTGTAATCCCAGCTACTC
Hairpin Sequence:
5'-CCGG-CGTCTGTAATCCCAGCTACTC-CTCGAG-GAGTAGCTGGGATTACAGACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTCTGTAATCCCAGCTACTCCTCGAGGAGTAGCTGGGATTACAGACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTCTGTAATCCCAGCTACTCCTCGAGGAGTAGCTGGGATTACAGACG-3'

Other clones with same target sequence:

(none)