Construct: shRNA TRCN0000256948

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003253.2-6656s21c1
DNA Barcode:
TAGTAGCTTTAGGAGTTATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TIAM1 (7074)
Gene Description:
TIAM Rac1 associated GEF 1
Transcript:
RefSeq NM_003253.2 (NON-CURRENT)
Match location:
Position 6656 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000256948)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAGTAGCTTTAGGAGTTATAT
Hairpin Sequence:
5'-CCGG-TAGTAGCTTTAGGAGTTATAT-CTCGAG-ATATAACTCCTAAAGCTACTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAGTAGCTTTAGGAGTTATATCTCGAGATATAACTCCTAAAGCTACTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAGTAGCTTTAGGAGTTATATCTCGAGATATAACTCCTAAAGCTACTA-3'

Other clones with same target sequence:

(none)