Construct: shRNA TRCN0000257015

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018469.3-3267s21c1
DNA Barcode:
GAACCTTGGCGGTCAACATTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TEX2 (55852)
Gene Description:
testis expressed 2
Transcript:
RefSeq NM_018469.3 (NON-CURRENT)
Match location:
Position 3267 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000257015)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACCTTGGCGGTCAACATTC
Hairpin Sequence:
5'-CCGG-GAACCTTGGCGGTCAACATTC-CTCGAG-GAATGTTGACCGCCAAGGTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACCTTGGCGGTCAACATTCCTCGAGGAATGTTGACCGCCAAGGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACCTTGGCGGTCAACATTCCTCGAGGAATGTTGACCGCCAAGGTTC-3'

Other clones with same target sequence:

(none)