Construct: shRNA TRCN0000257040

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081413.1-1404s21c1
DNA Barcode:
AGACCGCATCATCGCCATTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Unc13b (22249)
Gene Description:
unc-13 homolog B (C. elegans)
Transcript:
RefSeq NM_001081413.1 (NON-CURRENT)
Match location:
Position 1404 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000257040)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGACCGCATCATCGCCATTTA
Hairpin Sequence:
5'-CCGG-AGACCGCATCATCGCCATTTA-CTCGAG-TAAATGGCGATGATGCGGTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGACCGCATCATCGCCATTTACTCGAGTAAATGGCGATGATGCGGTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGACCGCATCATCGCCATTTACTCGAGTAAATGGCGATGATGCGGTCT-3'

Other clones with same target sequence:

(none)