Construct: shRNA TRCN0000257046

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_001474510.1-1338s21c1
DNA Barcode:
CAACACCAGAGACTCCGTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zkscan7 (382118)
Gene Description:
zinc finger with KRAB and SCAN domains 7
Transcript:
RefSeq XM_001474510.1 (NON-CURRENT)
Match location:
Position 1338 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000257046)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACACCAGAGACTCCGTAAT
Hairpin Sequence:
5'-CCGG-CAACACCAGAGACTCCGTAAT-CTCGAG-ATTACGGAGTCTCTGGTGTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACACCAGAGACTCCGTAATCTCGAGATTACGGAGTCTCTGGTGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACACCAGAGACTCCGTAATCTCGAGATTACGGAGTCTCTGGTGTTG-3'

Other clones with same target sequence:

(none)