Construct: shRNA TRCN0000257130

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001033352.3-1347s21c1
DNA Barcode:
GCAAGGAGACCATGGTGATAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Klhl21 (242785)
Gene Description:
kelch-like 21
Transcript:
RefSeq NM_001033352.3 (CURRENT)
Match location:
Position 1347 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000257130)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAGGAGACCATGGTGATAC
Hairpin Sequence:
5'-CCGG-GCAAGGAGACCATGGTGATAC-CTCGAG-GTATCACCATGGTCTCCTTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAGGAGACCATGGTGATACCTCGAGGTATCACCATGGTCTCCTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAGGAGACCATGGTGATACCTCGAGGTATCACCATGGTCTCCTTGC-3'

Other clones with same target sequence:

(none)