Construct: shRNA TRCN0000257135

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019867.2-741s21c1
DNA Barcode:
CCTCTGAGGCCTCCTACTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ngef (53972)
Gene Description:
neuronal guanine nucleotide exchange factor
Transcript:
RefSeq NM_019867.2 (CURRENT)
Match location:
Position 741 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000257135)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCTGAGGCCTCCTACTATA
Hairpin Sequence:
5'-CCGG-CCTCTGAGGCCTCCTACTATA-CTCGAG-TATAGTAGGAGGCCTCAGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCTGAGGCCTCCTACTATACTCGAGTATAGTAGGAGGCCTCAGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCTGAGGCCTCCTACTATACTCGAGTATAGTAGGAGGCCTCAGAGG-3'

Other clones with same target sequence:

(none)