Construct: shRNA TRCN0000257169

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001039522.1-1648s21c1
DNA Barcode:
CAGCGCACAGAGATGATTAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Leo1 (235497)
Gene Description:
Leo1, Paf1/RNA polymerase II complex component
Transcript:
RefSeq NM_001039522.1 (CURRENT)
Match location:
Position 1648 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000257169)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGCGCACAGAGATGATTAAG
Hairpin Sequence:
5'-CCGG-CAGCGCACAGAGATGATTAAG-CTCGAG-CTTAATCATCTCTGTGCGCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGCGCACAGAGATGATTAAGCTCGAGCTTAATCATCTCTGTGCGCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGCGCACAGAGATGATTAAGCTCGAGCTTAATCATCTCTGTGCGCTG-3'

Other clones with same target sequence:

(none)