Construct: shRNA TRCN0000257175

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002869.4-1130s21c1
DNA Barcode:
AGGAGGCTGTTCCTGCTAATC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RAB6A (5870)
Gene Description:
RAB6A, member RAS oncogene family
Transcript:
RefSeq NM_002869.4 (NON-CURRENT)
Match location:
Position 1130 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000257175)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGAGGCTGTTCCTGCTAATC
Hairpin Sequence:
5'-CCGG-AGGAGGCTGTTCCTGCTAATC-CTCGAG-GATTAGCAGGAACAGCCTCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGAGGCTGTTCCTGCTAATCCTCGAGGATTAGCAGGAACAGCCTCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGAGGCTGTTCCTGCTAATCCTCGAGGATTAGCAGGAACAGCCTCCT-3'

Other clones with same target sequence:

(none)