Construct: shRNA TRCN0000257216

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_080621.4-1362s21c1
DNA Barcode:
CCAAGCACTTTCATCATTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SAMD10 (140700)
Gene Description:
sterile alpha motif domain containing 10
Transcript:
RefSeq NM_080621.4 (NON-CURRENT)
Match location:
Position 1362 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000257216)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAGCACTTTCATCATTATT
Hairpin Sequence:
5'-CCGG-CCAAGCACTTTCATCATTATT-CTCGAG-AATAATGATGAAAGTGCTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAGCACTTTCATCATTATTCTCGAGAATAATGATGAAAGTGCTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAGCACTTTCATCATTATTCTCGAGAATAATGATGAAAGTGCTTGG-3'

Other clones with same target sequence:

  1. TRCN0000166912