Construct: shRNA TRCN0000257239

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_483996.3-2567s21c1
DNA Barcode:
AGATTGAGGGCACCGGTATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Usp34 (17847)
Gene Description:
ubiquitin specific peptidase 34
Transcript:
RefSeq XM_483996.3 (NON-CURRENT)
Match location:
Position 2567 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000257239)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGATTGAGGGCACCGGTATTA
Hairpin Sequence:
5'-CCGG-AGATTGAGGGCACCGGTATTA-CTCGAG-TAATACCGGTGCCCTCAATCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGATTGAGGGCACCGGTATTACTCGAGTAATACCGGTGCCCTCAATCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGATTGAGGGCACCGGTATTACTCGAGTAATACCGGTGCCCTCAATCT-3'

Other clones with same target sequence:

(none)