Construct: shRNA TRCN0000257276

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_001477831.1-1844s21c1
DNA Barcode:
GTGGTAAAGTTGTTGTTATAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm5165 (382161)
Gene Description:
predicted gene 5165
Transcript:
RefSeq XM_001477831.1 (NON-CURRENT)
Match location:
Position 1844 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000257276)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGGTAAAGTTGTTGTTATAG
Hairpin Sequence:
5'-CCGG-GTGGTAAAGTTGTTGTTATAG-CTCGAG-CTATAACAACAACTTTACCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGGTAAAGTTGTTGTTATAGCTCGAGCTATAACAACAACTTTACCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGGTAAAGTTGTTGTTATAGCTCGAGCTATAACAACAACTTTACCAC-3'

Other clones with same target sequence:

(none)