Construct: shRNA TRCN0000257332

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026106.4-846s21c1
DNA Barcode:
TTCTGAAGCCAACGAGATTTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dr1 (13486)
Gene Description:
down-regulator of transcription 1
Transcript:
RefSeq NM_026106.4 (CURRENT)
Match location:
Position 846 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000257332)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTCTGAAGCCAACGAGATTTG
Hairpin Sequence:
5'-CCGG-TTCTGAAGCCAACGAGATTTG-CTCGAG-CAAATCTCGTTGGCTTCAGAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTCTGAAGCCAACGAGATTTGCTCGAGCAAATCTCGTTGGCTTCAGAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTCTGAAGCCAACGAGATTTGCTCGAGCAAATCTCGTTGGCTTCAGAA-3'

Other clones with same target sequence:

(none)