Construct: shRNA TRCN0000257402

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004721.3-1757s21c1
DNA Barcode:
GCGGGAGAAGGAGCTCATTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP3K13 (9175)
Gene Description:
mitogen-activated protein kinase kinase kinase 13
Transcript:
RefSeq NM_004721.3 (NON-CURRENT)
Match location:
Position 1757 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000257402)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGGGAGAAGGAGCTCATTAA
Hairpin Sequence:
5'-CCGG-GCGGGAGAAGGAGCTCATTAA-CTCGAG-TTAATGAGCTCCTTCTCCCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGGGAGAAGGAGCTCATTAACTCGAGTTAATGAGCTCCTTCTCCCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGGGAGAAGGAGCTCATTAACTCGAGTTAATGAGCTCCTTCTCCCGC-3'

Other clones with same target sequence:

(none)