Construct: shRNA TRCN0000257562

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173038.3-403s21c1
DNA Barcode:
CGTGTCAGAACTCGATCTTTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tbcel (272589)
Gene Description:
tubulin folding cofactor E-like
Transcript:
RefSeq NM_173038.3 (CURRENT)
Match location:
Position 403 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000257562)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGTCAGAACTCGATCTTTC
Hairpin Sequence:
5'-CCGG-CGTGTCAGAACTCGATCTTTC-CTCGAG-GAAAGATCGAGTTCTGACACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGTCAGAACTCGATCTTTCCTCGAGGAAAGATCGAGTTCTGACACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGTCAGAACTCGATCTTTCCTCGAGGAAAGATCGAGTTCTGACACG-3'

Other clones with same target sequence:

(none)