Construct: shRNA TRCN0000257619

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133850.1-1177s21c1
DNA Barcode:
GCGACAAAGCATTCGAATATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Commd7 (99311)
Gene Description:
COMM domain containing 7
Transcript:
RefSeq NM_133850.1 (NON-CURRENT)
Match location:
Position 1177 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000257619)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGACAAAGCATTCGAATATA
Hairpin Sequence:
5'-CCGG-GCGACAAAGCATTCGAATATA-CTCGAG-TATATTCGAATGCTTTGTCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGACAAAGCATTCGAATATACTCGAGTATATTCGAATGCTTTGTCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGACAAAGCATTCGAATATACTCGAGTATATTCGAATGCTTTGTCGC-3'

Other clones with same target sequence:

(none)