Construct: shRNA TRCN0000257752

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020705.1-1391s21c1
DNA Barcode:
CTTCTTTGGGACCGGAGAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TBC1D24 (57465)
Gene Description:
TBC1 domain family member 24
Transcript:
RefSeq NM_020705.1 (NON-CURRENT)
Match location:
Position 1391 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000257752)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCTTTGGGACCGGAGAATG
Hairpin Sequence:
5'-CCGG-CTTCTTTGGGACCGGAGAATG-CTCGAG-CATTCTCCGGTCCCAAAGAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCTTTGGGACCGGAGAATGCTCGAGCATTCTCCGGTCCCAAAGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCTTTGGGACCGGAGAATGCTCGAGCATTCTCCGGTCCCAAAGAAG-3'

Other clones with same target sequence:

(none)