Construct: shRNA TRCN0000257758

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025416.2-918s21c1
DNA Barcode:
GATGACTCAGTGGCCACATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Them5 (66198)
Gene Description:
thioesterase superfamily member 5
Transcript:
RefSeq NM_025416.2 (NON-CURRENT)
Match location:
Position 918 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000257758)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGACTCAGTGGCCACATAA
Hairpin Sequence:
5'-CCGG-GATGACTCAGTGGCCACATAA-CTCGAG-TTATGTGGCCACTGAGTCATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGACTCAGTGGCCACATAACTCGAGTTATGTGGCCACTGAGTCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGACTCAGTGGCCACATAACTCGAGTTATGTGGCCACTGAGTCATC-3'

Other clones with same target sequence:

(none)