Construct: shRNA TRCN0000257790
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_172614.3-358s21c1
- DNA Barcode:
- CTACCTAGCAGCTGTCGATTT
Original Target:
- Taxon:
- Mus musculus (mouse)
- Gene:
- Tmem44 (224090)
- Gene Description:
- transmembrane protein 44
- Transcript:
- RefSeq NM_172614.3 (CURRENT)
- Match location:
- Position 358 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000257790)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
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Sequence Information
- Target Sequence:
- CTACCTAGCAGCTGTCGATTT
- Hairpin Sequence:
- 5'-CCGG-CTACCTAGCAGCTGTCGATTT-CTCGAG-AAATCGACAGCTGCTAGGTAG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCTACCTAGCAGCTGTCGATTTCTCGAGAAATCGACAGCTGCTAGGTAGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACTACCTAGCAGCTGTCGATTTCTCGAGAAATCGACAGCTGCTAGGTAG-3'