Construct: shRNA TRCN0000257792

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178184.1-100s21c1
DNA Barcode:
CTGCTCCGCAAGGGCAACTAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hist1h2an (319170)
Gene Description:
histone cluster 1, H2an
Transcript:
RefSeq NM_178184.1 (NON-CURRENT)
Match location:
Position 100 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000257792)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGCTCCGCAAGGGCAACTAC
Hairpin Sequence:
5'-CCGG-CTGCTCCGCAAGGGCAACTAC-CTCGAG-GTAGTTGCCCTTGCGGAGCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGCTCCGCAAGGGCAACTACCTCGAGGTAGTTGCCCTTGCGGAGCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGCTCCGCAAGGGCAACTACCTCGAGGTAGTTGCCCTTGCGGAGCAG-3'

Other clones with same target sequence:

  1. TRCN0000369944