Construct: shRNA TRCN0000257843

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153134.3-617s21c1
DNA Barcode:
GATTCCCAGAATGCAGCTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Irgq (210146)
Gene Description:
immunity-related GTPase family, Q
Transcript:
RefSeq NM_153134.3 (CURRENT)
Match location:
Position 617 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000257843)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATTCCCAGAATGCAGCTAAA
Hairpin Sequence:
5'-CCGG-GATTCCCAGAATGCAGCTAAA-CTCGAG-TTTAGCTGCATTCTGGGAATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATTCCCAGAATGCAGCTAAACTCGAGTTTAGCTGCATTCTGGGAATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATTCCCAGAATGCAGCTAAACTCGAGTTTAGCTGCATTCTGGGAATC-3'

Other clones with same target sequence:

(none)