Construct: shRNA TRCN0000257849

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011727.2-509s21c1
DNA Barcode:
TATGGAGCAACAGAAGTTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Xlr3c (22446)
Gene Description:
X-linked lymphocyte-regulated 3C
Transcript:
RefSeq NM_011727.2 (CURRENT)
Match location:
Position 509 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000257849)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TATGGAGCAACAGAAGTTTAT
Hairpin Sequence:
5'-CCGG-TATGGAGCAACAGAAGTTTAT-CTCGAG-ATAAACTTCTGTTGCTCCATA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTATGGAGCAACAGAAGTTTATCTCGAGATAAACTTCTGTTGCTCCATATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATATGGAGCAACAGAAGTTTATCTCGAGATAAACTTCTGTTGCTCCATA-3'

Other clones with same target sequence:

(none)