Construct: shRNA TRCN0000257850

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178621.3-1684s21c1
DNA Barcode:
TAATTGCAGCATGAGTTAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Phyhipl (70911)
Gene Description:
phytanoyl-CoA hydroxylase interacting protein-like
Transcript:
RefSeq NM_178621.3 (NON-CURRENT)
Match location:
Position 1684 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000257850)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAATTGCAGCATGAGTTAAAT
Hairpin Sequence:
5'-CCGG-TAATTGCAGCATGAGTTAAAT-CTCGAG-ATTTAACTCATGCTGCAATTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAATTGCAGCATGAGTTAAATCTCGAGATTTAACTCATGCTGCAATTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAATTGCAGCATGAGTTAAATCTCGAGATTTAACTCATGCTGCAATTA-3'

Other clones with same target sequence:

(none)