Construct: shRNA TRCN0000257881

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178256.3-428s21c1
DNA Barcode:
GTGAGAACAGAGAGTATTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Reps2 (194590)
Gene Description:
RALBP1 associated Eps domain containing protein 2
Transcript:
RefSeq NM_178256.3 (NON-CURRENT)
Match location:
Position 428 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000257881)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGAGAACAGAGAGTATTAAA
Hairpin Sequence:
5'-CCGG-GTGAGAACAGAGAGTATTAAA-CTCGAG-TTTAATACTCTCTGTTCTCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGAGAACAGAGAGTATTAAACTCGAGTTTAATACTCTCTGTTCTCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGAGAACAGAGAGTATTAAACTCGAGTTTAATACTCTCTGTTCTCAC-3'

Other clones with same target sequence:

(none)