Construct: shRNA TRCN0000257992

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178731.4-1771s21c1
DNA Barcode:
ACCATGGATATATCGGTTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lrrtm4 (243499)
Gene Description:
leucine rich repeat transmembrane neuronal 4
Transcript:
RefSeq NM_178731.4 (NON-CURRENT)
Match location:
Position 1771 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000257992)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCATGGATATATCGGTTAAT
Hairpin Sequence:
5'-CCGG-ACCATGGATATATCGGTTAAT-CTCGAG-ATTAACCGATATATCCATGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCATGGATATATCGGTTAATCTCGAGATTAACCGATATATCCATGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCATGGATATATCGGTTAATCTCGAGATTAACCGATATATCCATGGT-3'

Other clones with same target sequence:

(none)