Construct: shRNA TRCN0000258041

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029791.3-800s21c1
DNA Barcode:
AGAGGATGCCATCCGGCTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Bicd2 (76895)
Gene Description:
bicaudal D homolog 2 (Drosophila)
Transcript:
RefSeq NM_029791.3 (NON-CURRENT)
Match location:
Position 800 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000258041)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAGGATGCCATCCGGCTTAA
Hairpin Sequence:
5'-CCGG-AGAGGATGCCATCCGGCTTAA-CTCGAG-TTAAGCCGGATGGCATCCTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAGGATGCCATCCGGCTTAACTCGAGTTAAGCCGGATGGCATCCTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAGGATGCCATCCGGCTTAACTCGAGTTAAGCCGGATGGCATCCTCT-3'

Other clones with same target sequence:

(none)