Construct: shRNA TRCN0000258092

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028839.3-452s21c1
DNA Barcode:
GTAGGCATGCTGCTCATTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tmem110 (69179)
Gene Description:
transmembrane protein 110
Transcript:
RefSeq NM_028839.3 (NON-CURRENT)
Match location:
Position 452 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000258092)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTAGGCATGCTGCTCATTTAT
Hairpin Sequence:
5'-CCGG-GTAGGCATGCTGCTCATTTAT-CTCGAG-ATAAATGAGCAGCATGCCTAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTAGGCATGCTGCTCATTTATCTCGAGATAAATGAGCAGCATGCCTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTAGGCATGCTGCTCATTTATCTCGAGATAAATGAGCAGCATGCCTAC-3'

Other clones with same target sequence:

  1. TRCN0000216810