Construct: shRNA TRCN0000258095

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001042592.2-1030s21c1
DNA Barcode:
GGACTACTCCTTAGCTGTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Arrdc4 (66412)
Gene Description:
arrestin domain containing 4
Transcript:
RefSeq NM_001042592.2 (CURRENT)
Match location:
Position 1030 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000258095)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGACTACTCCTTAGCTGTATA
Hairpin Sequence:
5'-CCGG-GGACTACTCCTTAGCTGTATA-CTCGAG-TATACAGCTAAGGAGTAGTCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGACTACTCCTTAGCTGTATACTCGAGTATACAGCTAAGGAGTAGTCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGACTACTCCTTAGCTGTATACTCGAGTATACAGCTAAGGAGTAGTCC-3'

Other clones with same target sequence:

(none)