Construct: shRNA TRCN0000258099

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153409.4-1329s21c1
DNA Barcode:
TCGCAGACAATTTCGAGATTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Csrnp3 (77771)
Gene Description:
cysteine-serine-rich nuclear protein 3
Transcript:
RefSeq NM_153409.4 (NON-CURRENT)
Match location:
Position 1329 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000258099)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCGCAGACAATTTCGAGATTG
Hairpin Sequence:
5'-CCGG-TCGCAGACAATTTCGAGATTG-CTCGAG-CAATCTCGAAATTGTCTGCGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCGCAGACAATTTCGAGATTGCTCGAGCAATCTCGAAATTGTCTGCGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCGCAGACAATTTCGAGATTGCTCGAGCAATCTCGAAATTGTCTGCGA-3'

Other clones with same target sequence:

(none)