Construct: shRNA TRCN0000258101

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028887.3-639s21c1
DNA Barcode:
GTCAGCTACTTCTCGAAATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Smchd1 (74355)
Gene Description:
SMC hinge domain containing 1
Transcript:
RefSeq NM_028887.3 (NON-CURRENT)
Match location:
Position 639 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000258101)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCAGCTACTTCTCGAAATAA
Hairpin Sequence:
5'-CCGG-GTCAGCTACTTCTCGAAATAA-CTCGAG-TTATTTCGAGAAGTAGCTGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCAGCTACTTCTCGAAATAACTCGAGTTATTTCGAGAAGTAGCTGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCAGCTACTTCTCGAAATAACTCGAGTTATTTCGAGAAGTAGCTGAC-3'

Other clones with same target sequence:

  1. TRCN0000217618