Construct: shRNA TRCN0000258156

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001039114.1-2437s21c1
DNA Barcode:
ACAAGCCTTCCAGGCGTAAGT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Acsbg2 (328845)
Gene Description:
acyl-CoA synthetase bubblegum family member 2
Transcript:
RefSeq NM_001039114.1 (CURRENT)
Match location:
Position 2437 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000258156)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACAAGCCTTCCAGGCGTAAGT
Hairpin Sequence:
5'-CCGG-ACAAGCCTTCCAGGCGTAAGT-CTCGAG-ACTTACGCCTGGAAGGCTTGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACAAGCCTTCCAGGCGTAAGTCTCGAGACTTACGCCTGGAAGGCTTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACAAGCCTTCCAGGCGTAAGTCTCGAGACTTACGCCTGGAAGGCTTGT-3'

Other clones with same target sequence:

(none)