Construct: shRNA TRCN0000258238

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001085515.1-1319s21c1
DNA Barcode:
CATCCGTTTGTCAACTATAAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
AI464131 (329828)
Gene Description:
expressed sequence AI464131
Transcript:
RefSeq NM_001085515.1 (NON-CURRENT)
Match location:
Position 1319 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000258238)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATCCGTTTGTCAACTATAAC
Hairpin Sequence:
5'-CCGG-CATCCGTTTGTCAACTATAAC-CTCGAG-GTTATAGTTGACAAACGGATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATCCGTTTGTCAACTATAACCTCGAGGTTATAGTTGACAAACGGATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATCCGTTTGTCAACTATAACCTCGAGGTTATAGTTGACAAACGGATG-3'

Other clones with same target sequence:

(none)