Construct: shRNA TRCN0000262096

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001110227.1-1011s21c1
DNA Barcode:
ATCATGTTACATCACCGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kcnj13 (100040591)
Gene Description:
potassium inwardly-rectifying channel, subfamily J, member 13
Transcript:
RefSeq NM_001110227.1 (NON-CURRENT)
Match location:
Position 1011 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000262096)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCATGTTACATCACCGATTT
Hairpin Sequence:
5'-CCGG-ATCATGTTACATCACCGATTT-CTCGAG-AAATCGGTGATGTAACATGAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCATGTTACATCACCGATTTCTCGAGAAATCGGTGATGTAACATGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCATGTTACATCACCGATTTCTCGAGAAATCGGTGATGTAACATGAT-3'

Other clones with same target sequence:

(none)