Construct: shRNA TRCN0000262101

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001100181.1-380s21c1
DNA Barcode:
GCTAATGGTATCTACAGATTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cyp4a32 (100040843)
Gene Description:
cytochrome P450, family 4, subfamily a, polypeptide 32
Transcript:
RefSeq NM_001100181.1 (CURRENT)
Match location:
Position 380 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000262101)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTAATGGTATCTACAGATTG
Hairpin Sequence:
5'-CCGG-GCTAATGGTATCTACAGATTG-CTCGAG-CAATCTGTAGATACCATTAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTAATGGTATCTACAGATTGCTCGAGCAATCTGTAGATACCATTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTAATGGTATCTACAGATTGCTCGAGCAATCTGTAGATACCATTAGC-3'

Other clones with same target sequence:

(none)