Construct: shRNA TRCN0000262117
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_001045513.2-1752s21c1
- DNA Barcode:
- AGACTGCTGCCTGGCATTAAA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Raph1 (77300)
- Gene Description:
- Ras association (RalGDS/AF-6) and pleckstrin homology domains 1
- Transcript:
- RefSeq NM_001045513.2 (NON-CURRENT)
- Match location:
- Position 1752 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000262117)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- AGACTGCTGCCTGGCATTAAA
- Hairpin Sequence:
-
5'-CCGG-AGACTGCTGCCTGGCATTAAA-CTCGAG-TTTAATGCCAGGCAGCAGTCT-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGAGACTGCTGCCTGGCATTAAACTCGAGTTTAATGCCAGGCAGCAGTCTTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAAGACTGCTGCCTGGCATTAAACTCGAGTTTAATGCCAGGCAGCAGTCT-3'
Other clones with same target sequence:
(none)
