Construct: shRNA TRCN0000262129

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001104946.2-177s21c1
DNA Barcode:
ATTACTTCTTGACTATGATTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm2012 (100039030)
Gene Description:
predicted gene 2012
Transcript:
RefSeq NM_001104946.2 (CURRENT)
Match location:
Position 177 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000262129)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATTACTTCTTGACTATGATTC
Hairpin Sequence:
5'-CCGG-ATTACTTCTTGACTATGATTC-CTCGAG-GAATCATAGTCAAGAAGTAAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATTACTTCTTGACTATGATTCCTCGAGGAATCATAGTCAAGAAGTAATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATTACTTCTTGACTATGATTCCTCGAGGAATCATAGTCAAGAAGTAAT-3'

Other clones with same target sequence:

(none)