Construct: shRNA TRCN0000262184

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001080790.1-492s21c1
DNA Barcode:
ATTTCCGTCTACTACACTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAGEA9B (728269)
Gene Description:
MAGE family member A9B
Transcript:
RefSeq NM_001080790.1 (CURRENT)
Match location:
Position 492 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000262184)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATTTCCGTCTACTACACTTTA
Hairpin Sequence:
5'-CCGG-ATTTCCGTCTACTACACTTTA-CTCGAG-TAAAGTGTAGTAGACGGAAAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATTTCCGTCTACTACACTTTACTCGAGTAAAGTGTAGTAGACGGAAATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATTTCCGTCTACTACACTTTACTCGAGTAAAGTGTAGTAGACGGAAAT-3'

Other clones with same target sequence:

(none)