Construct: shRNA TRCN0000262274

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033590.3-1661s21c1
DNA Barcode:
CACTTGGATCCTTCGACTATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pcdhga7 (93715)
Gene Description:
protocadherin gamma subfamily A, 7
Transcript:
RefSeq NM_033590.3 (CURRENT)
Match location:
Position 1661 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000262274)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACTTGGATCCTTCGACTATG
Hairpin Sequence:
5'-CCGG-CACTTGGATCCTTCGACTATG-CTCGAG-CATAGTCGAAGGATCCAAGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACTTGGATCCTTCGACTATGCTCGAGCATAGTCGAAGGATCCAAGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACTTGGATCCTTCGACTATGCTCGAGCATAGTCGAAGGATCCAAGTG-3'

Other clones with same target sequence:

(none)