Construct: shRNA TRCN0000262283

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001017438.1-417s21c1
DNA Barcode:
CTTATGACAGGACATGCTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CT45A6 (541465)
Gene Description:
cancer/testis antigen family 45 member A6
Transcript:
RefSeq NM_001017438.1 (NON-CURRENT)
Match location:
Position 417 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000262283)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTATGACAGGACATGCTATT
Hairpin Sequence:
5'-CCGG-CTTATGACAGGACATGCTATT-CTCGAG-AATAGCATGTCCTGTCATAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTATGACAGGACATGCTATTCTCGAGAATAGCATGTCCTGTCATAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTATGACAGGACATGCTATTCTCGAGAATAGCATGTCCTGTCATAAG-3'

Other clones with same target sequence:

(none)