Construct: shRNA TRCN0000262295

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198945.3-2340s21c1
DNA Barcode:
TCGGAAGAGTTGGACCTTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NBEAL1 (65065)
Gene Description:
neurobeachin like 1
Transcript:
RefSeq NM_198945.3 (NON-CURRENT)
Match location:
Position 2340 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000262295)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCGGAAGAGTTGGACCTTAAT
Hairpin Sequence:
5'-CCGG-TCGGAAGAGTTGGACCTTAAT-CTCGAG-ATTAAGGTCCAACTCTTCCGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCGGAAGAGTTGGACCTTAATCTCGAGATTAAGGTCCAACTCTTCCGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCGGAAGAGTTGGACCTTAATCTCGAGATTAAGGTCCAACTCTTCCGA-3'

Other clones with same target sequence:

(none)