Construct: shRNA TRCN0000262301

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001079530.1-476s21c1
DNA Barcode:
GAGAAACATAACGGCGGTAGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CFC1B (653275)
Gene Description:
cripto, FRL-1, cryptic family 1B
Transcript:
RefSeq NM_001079530.1 (NON-CURRENT)
Match location:
Position 476 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000262301)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGAAACATAACGGCGGTAGA
Hairpin Sequence:
5'-CCGG-GAGAAACATAACGGCGGTAGA-CTCGAG-TCTACCGCCGTTATGTTTCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGAAACATAACGGCGGTAGACTCGAGTCTACCGCCGTTATGTTTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGAAACATAACGGCGGTAGACTCGAGTCTACCGCCGTTATGTTTCTC-3'

Other clones with same target sequence:

(none)