Construct: shRNA TRCN0000262323

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_078487.2-2246s21c1
DNA Barcode:
TCCAGGGTTGCTTGATCATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDKN2B (1030)
Gene Description:
cyclin dependent kinase inhibitor 2B
Transcript:
RefSeq NM_078487.2 (CURRENT)
Match location:
Position 2246 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000262323)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCAGGGTTGCTTGATCATTA
Hairpin Sequence:
5'-CCGG-TCCAGGGTTGCTTGATCATTA-CTCGAG-TAATGATCAAGCAACCCTGGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCAGGGTTGCTTGATCATTACTCGAGTAATGATCAAGCAACCCTGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCAGGGTTGCTTGATCATTACTCGAGTAATGATCAAGCAACCCTGGA-3'

Other clones with same target sequence:

(none)