Construct: shRNA TRCN0000262397

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001126334.1-1804s21c1
DNA Barcode:
TTCAGCATTGAGAGTATTATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FOXD4L5 (653427)
Gene Description:
forkhead box D4 like 5
Transcript:
RefSeq NM_001126334.1 (CURRENT)
Match location:
Position 1804 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000262397)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTCAGCATTGAGAGTATTATG
Hairpin Sequence:
5'-CCGG-TTCAGCATTGAGAGTATTATG-CTCGAG-CATAATACTCTCAATGCTGAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTCAGCATTGAGAGTATTATGCTCGAGCATAATACTCTCAATGCTGAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTCAGCATTGAGAGTATTATGCTCGAGCATAATACTCTCAATGCTGAA-3'

Other clones with same target sequence:

(none)