Construct: shRNA TRCN0000262466

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024897.2-1496s21c1
DNA Barcode:
CATCTCAGCCTGGAACCAACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PAQR6 (79957)
Gene Description:
progestin and adipoQ receptor family member 6
Transcript:
RefSeq NM_024897.2 (NON-CURRENT)
Match location:
Position 1496 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000262466)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATCTCAGCCTGGAACCAACA
Hairpin Sequence:
5'-CCGG-CATCTCAGCCTGGAACCAACA-CTCGAG-TGTTGGTTCCAGGCTGAGATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATCTCAGCCTGGAACCAACACTCGAGTGTTGGTTCCAGGCTGAGATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATCTCAGCCTGGAACCAACACTCGAGTGTTGGTTCCAGGCTGAGATG-3'

Other clones with same target sequence:

(none)