Construct: shRNA TRCN0000262509

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001025231.1-953s21c1
DNA Barcode:
CGCCGCTCTGAACCCATATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KPRP (448834)
Gene Description:
keratinocyte proline rich protein
Transcript:
RefSeq NM_001025231.1 (NON-CURRENT)
Match location:
Position 953 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000262509)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCCGCTCTGAACCCATATAT
Hairpin Sequence:
5'-CCGG-CGCCGCTCTGAACCCATATAT-CTCGAG-ATATATGGGTTCAGAGCGGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCCGCTCTGAACCCATATATCTCGAGATATATGGGTTCAGAGCGGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCCGCTCTGAACCCATATATCTCGAGATATATGGGTTCAGAGCGGCG-3'

Other clones with same target sequence:

(none)