Construct: shRNA TRCN0000262538

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_201645.2-170s21c1
DNA Barcode:
CTGAGTATGCTTGGAGTTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ugt1a1 (394436)
Gene Description:
UDP glucuronosyltransferase 1 family, polypeptide A1
Transcript:
RefSeq NM_201645.2 (CURRENT)
Match location:
Position 170 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000262538)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGAGTATGCTTGGAGTTATT
Hairpin Sequence:
5'-CCGG-CTGAGTATGCTTGGAGTTATT-CTCGAG-AATAACTCCAAGCATACTCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGAGTATGCTTGGAGTTATTCTCGAGAATAACTCCAAGCATACTCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGAGTATGCTTGGAGTTATTCTCGAGAATAACTCCAAGCATACTCAG-3'

Other clones with same target sequence:

(none)