Construct: shRNA TRCN0000262549

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024274.2-1226s21c1
DNA Barcode:
GAGTTATTTGCTGGCGTAAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fars2 (69955)
Gene Description:
phenylalanine-tRNA synthetase 2 (mitochondrial)
Transcript:
RefSeq NM_024274.2 (NON-CURRENT)
Match location:
Position 1226 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000262549)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGTTATTTGCTGGCGTAAAG
Hairpin Sequence:
5'-CCGG-GAGTTATTTGCTGGCGTAAAG-CTCGAG-CTTTACGCCAGCAAATAACTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGTTATTTGCTGGCGTAAAGCTCGAGCTTTACGCCAGCAAATAACTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGTTATTTGCTGGCGTAAAGCTCGAGCTTTACGCCAGCAAATAACTC-3'

Other clones with same target sequence:

(none)