Construct: shRNA TRCN0000262595

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012414.3-5711s21c1
DNA Barcode:
GTATGTCCTTCAGGGATATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RAB3GAP2 (25782)
Gene Description:
RAB3 GTPase activating non-catalytic protein subunit 2
Transcript:
RefSeq NM_012414.3 (NON-CURRENT)
Match location:
Position 5711 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000262595)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTATGTCCTTCAGGGATATAA
Hairpin Sequence:
5'-CCGG-GTATGTCCTTCAGGGATATAA-CTCGAG-TTATATCCCTGAAGGACATAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTATGTCCTTCAGGGATATAACTCGAGTTATATCCCTGAAGGACATACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTATGTCCTTCAGGGATATAACTCGAGTTATATCCCTGAAGGACATAC-3'

Other clones with same target sequence:

(none)