Construct: shRNA TRCN0000262626

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001039840.2-369s21c1
DNA Barcode:
GAGGAGCATCTGCGGAGATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CHIC1 (53344)
Gene Description:
cysteine rich hydrophobic domain 1
Transcript:
RefSeq NM_001039840.2 (NON-CURRENT)
Match location:
Position 369 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000262626)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGGAGCATCTGCGGAGATAT
Hairpin Sequence:
5'-CCGG-GAGGAGCATCTGCGGAGATAT-CTCGAG-ATATCTCCGCAGATGCTCCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGGAGCATCTGCGGAGATATCTCGAGATATCTCCGCAGATGCTCCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGGAGCATCTGCGGAGATATCTCGAGATATCTCCGCAGATGCTCCTC-3'

Other clones with same target sequence:

(none)