Construct: shRNA TRCN0000262655

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021933.2-661s21c1
DNA Barcode:
GAGGAGTCTGCAGTTCCTAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MIIP (60672)
Gene Description:
migration and invasion inhibitory protein
Transcript:
RefSeq NM_021933.2 (NON-CURRENT)
Match location:
Position 661 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000262655)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGGAGTCTGCAGTTCCTAAG
Hairpin Sequence:
5'-CCGG-GAGGAGTCTGCAGTTCCTAAG-CTCGAG-CTTAGGAACTGCAGACTCCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGGAGTCTGCAGTTCCTAAGCTCGAGCTTAGGAACTGCAGACTCCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGGAGTCTGCAGTTCCTAAGCTCGAGCTTAGGAACTGCAGACTCCTC-3'

Other clones with same target sequence:

(none)